Rankings
| # | Team | MP | W | D | L | F | A | G | P |
|---|---|---|---|---|---|---|---|---|---|
| 1 | Cincinnati | 31 | 19 | 8 | 4 | 53 | 35 | +18 | 65 |
| 2 |  Orlando City |
31 | 15 | 9 | 7 | 49 | 37 | +12 | 54 |
| 3 |  New England |
30 | 14 | 10 | 6 | 51 | 37 | +14 | 52 |
| 4 |  Philadelphia Union |
31 | 14 | 9 | 8 | 53 | 37 | +16 | 51 |
| 5 |  Columbus Crew |
31 | 14 | 8 | 9 | 62 | 43 | +19 | 50 |
| 6 | Atlanta United | 31 | 13 | 10 | 8 | 61 | 47 | +14 | 49 |
| 7 | Nashville SC | 30 | 12 | 9 | 9 | 36 | 28 | +8 | 45 |
| 8 |  New York City |
32 | 8 | 14 | 10 | 34 | 37 | -3 | 38 |
| 9 | Montréal | 31 | 11 | 4 | 16 | 30 | 48 | -18 | 37 |
| 10 | D.C. United | 32 | 9 | 10 | 13 | 43 | 46 | -3 | 37 |
| 11 |  Chicago Fire |
31 | 9 | 10 | 12 | 35 | 47 | -12 | 37 |
| 12 |  New York RB |
31 | 8 | 10 | 13 | 30 | 38 | -8 | 34 |
| 13 | Inter Miami | 30 | 9 | 6 | 15 | 38 | 46 | -8 | 33 |
| 14 | Charlotte | 30 | 7 | 12 | 11 | 37 | 50 | -13 | 33 |
| 15 |  Toronto |
31 | 4 | 10 | 17 | 26 | 51 | -25 | 22 |
Fixtures
| October 4, 2023 | ||
| Charlotte | 23:30 | Toronto |
| Cincinnati | 23:30 | New York RB |
| Montréal | 23:30 |  Houston Dynamo |
| New England |
23:30 | Columbus Crew |
| Philadelphia Union |
23:30 | Atlanta United |
| October 5, 2023 | ||
| Austin | 00:30 | D.C. United |
| Chicago Fire |
00:30 | Inter Miami |
Dallas  |
00:30 |  Colorado Rapids |
| Nashville SC | 00:30 | Orlando City |
| Vancouver Whitecaps FC | 02:30 | St. Louis City |
Seattle Sounders  |
02:30 |  LA Galaxy |
| Los Angeles | 02:30 | Minnesota United |
Results
| October 2, 2023 | ||
| Los Angeles | 0 - 1 |  Real Salt Lake |
| October 1, 2023 | ||
| Vancouver Whitecaps FC | 2 - 2 | D.C. United |
| LA Galaxy |
3 - 3 |  Portland Timbers |
| Colorado Rapids |
1 - 0 | Austin |
| Inter Miami | 1 - 1 | New York City |
| St. Louis City | 4 - 1 |  Sporting KC |
| Nashville SC | 0 - 0 | Seattle Sounders |
| Minnesota United | 1 - 1 |  SJ Earthquakes |
| Houston Dynamo |
0 - 0 | Dallas |
| September 30, 2023 | ||
| New England |
2 - 1 | Charlotte |
| Columbus Crew |
1 - 1 | Philadelphia Union |
| Orlando City |
3 - 0 | Montréal |
| New York RB |
0 - 1 | Chicago Fire |
| Toronto |
2 - 3 | Cincinnati |
| September 28, 2023 | ||
| Colorado Rapids |
2 - 2 | Vancouver Whitecaps FC |
| September 27, 2023 | ||
| Philadelphia Union |
1 - 1 | Dallas |
| September 25, 2023 | ||
| Austin | 3 - 3 | LA Galaxy |
| September 24, 2023 | ||
| Orlando City |
1 - 1 | Inter Miami |